Abstract: This paper mainly introduces the measurement principle of 722 spectrophotometer, the specification and main technical parameters of 722 spectrophotometer and the composition of 722 visible spectrophotometer. Finally, the steps and precautions of the 722 spectrophotometer are introduced in detail.
722 spectrophotometer measurement principleThe theoretical basis for the spectrophotometric measurement is the Bolang-Beier law: when the substance in the liquid is irradiated and excited by light, it produces an effect on light absorption. However, the absorption of light by matter is selective, and various substances have their own absorption spectra. Therefore, according to the law, when a bundle of monochromatic light passes through a rare color solution of a certain concentration range, the absorption degree A of the solution is proportional to the concentration c (g/l) of the solution or the thickness b (cm) of the liquid layer. Its law expression A=abc
(a is the proportionality factor). When the unit of c is mol/l, the proportional coefficient is represented by ε, and A = εbc is called the molar absorptivity. Its unit is L·mol-1·cm-1, which is the characteristic constant of a colored substance at a certain wavelength.
When T (light transmittance) = I / I0A (absorbance) = - lgT or A = K · C · L (thickness of the cuvette), when the incident light I, the absorption coefficient, and the optical path length of the solution are constant, The transmitted light varies depending on the concentration of the solution, that is, "K" is a constant. The thickness of the cuvette is constant, and "L" and "I0" are also fixed. As long as A is measured, "C" can be calculated.
722 spectrophotometer specifications and main technical parameters
The instrument is mainly composed of a light source, a monochromator, a sample chamber, a detector, a signal processor and a display.
722 series visible spectrophotometer appearance 
722 series visible spectrophotometer top view 
722 series visible spectrophotometer rear view 
722 Series Visible Spectrophotometer Button Description
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