Recently, some students asked about the comparison of paraffin sections and frozen sections in the immunohistochemistry kit. Shanghai Jinma Bio is mainly engaged in the elisa kit, and it is known about immunohistochemistry. The following is a reference for the technical staff to give advice. Welcome to read, please contact the professional for specific questions. Let's interpret these two points together with Xiaobian!
(1) It is not necessary to make paraffin sections for frozen sections. This is the conclusion I asked from a teacher today. Because high temperature baking of the paraffin section may destroy the antigenicity of the tissue. If the antigenicity of the tissue is stable, it can be used for paraffin sectioning; however, if it is required to be paraffin-sliced, it can be used for frozen sectioning.
(2) The advantage of frozen section is that it can better preserve the antigen immunological activity of the tissue, and does not require antigen retrieval in immunohistochemistry. The disadvantage is that the cells are easy to form ice crystals and destroy the cell structure, which may cause the antigen to disperse; the thickness of the slice is thicker than that of the paraffin, and the film is not as beautiful as paraffin. When you buy a primary antibody, the catalogue says what kind of slice to make. If it says that it can only be frozen, you can't make paraffin. If you write both, you can do it.
(3) The advantages of paraffin section can maintain the morphological structure of tissue cells, and easy to store at room temperature, while frozen sections are more troublesome, must exist in a low-temperature refrigerator of -80 degrees, especially for in situ hybridization. In order to prevent RNA degradation, preservation has always been important. Since the paraffin section can be cut to about 4 microns, the in situ hybridization probe is easy to penetrate into the tissue, is easy to succeed, and the color/morphology obtained is better than that of the frozen section.
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