Our technology is against the proposal. At the same time, it is also clear that our sales staff is advised to communicate with the customer before the sale. It is recommended that the teacher's whole blood sample be extracted and the serum or plasma should be centrifuged in time. It is required to be stored at -70 °C. If it can be carried out for about one month, it can be stored at -20 °C, and it cannot be frozen and thawed repeatedly. As for the whole blood after cryopreservation, it is not possible to extract serum and plasma samples.
Elisa kit
The specific reasons are as follows:
ELISA kit experiment novice customers will consult me ​​this practice
The cause is that after the whole blood is frozen and thawed, it is still possible to extract the serum by conventional centrifugation. The only unfavorable point is that a relatively serious hemolysis event will occur along with the process of freezing and thawing. And can the last extracted serum be done?
ELISA kit
It depends mainly on the degree of hemolysis, the nature of the target protein you are testing and the ultimate goal of your experiment.
Reason 2
The intracellular components released by hemolysis tend to affect the background and even false positives; while the release of proteases may lead to the degradation of the target protein, ultimately affecting the results; the size, stability, specificity (relative to antibodies) of the target protein affects Its own adsorption with the enzyme plate, self-degradation, self-antibody binding rate and so on. In the presence of various uncertainties, if you are only doing qualitative testing of certain serum proteins in serum, do it.
ELISA kit
There should be little problem; if it is quantitative analysis, this approach is not recommended from a reliability point of view.
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